NucleoBond PC 2000 EF, Mega kit for endotoxin-free plasmid DNA

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Delivery time approx. 5 working days

740549 031010410001
Package unit 5 Preps
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1st generation anion exchange technology for endotoxin-free plasmid DNA more

1st generation anion exchange technology for endotoxin-free plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Mega prep
Handling Gravity flow
Lysate clarification Bottle top filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 150−500 mL
Vector size < 300 kbp
Typical yield 500−2000 µg
Theoretical binding capacity 2000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 150 min/2 preps
Typical downstream application Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Scope of delivery NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A
Gross weight (incl. packaging) 3985.1 g / 8.79 lbs
Packaging dimensions 280 x 245 x 310 mm / 11.02 x 9.65 x 12.20 Inch
Hazardous material Yes
Resuspension Buffer S1-EF Resuspension Buffer S1-EF
Content 1000 mL
REF 740790.1
Lysis Buffer S2-EF Lysis Buffer S2-EF
Content 1000 mL
REF 740791.1
Neutralization Buffer S3-EF Neutralization Buffer S3-EF
Content 1000 mL
REF 740792.1
Equilibration Buffer N2-EF Equilibration Buffer N2-EF
Content 1000 mL
REF 740793.1
Wash Buffer N3-EF Wash Buffer N3-EF
Content 1000 mL
REF 740794.1
Wash Buffer N4-EF Wash Buffer N4-EF
Content 1000 mL
REF 740795.1
Elution Buffer N5-EF Elution Buffer N5-EF
Content 600 mL
REF 740796.1
Redissolving Buffer TE-EF Redissolving Buffer TE-EF
Content 1000 mL
REF 740797.1