NucleoBond PC 2000, Mega kit for transfection-grade plasmid DNA

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Delivery time approx. 5 working days

740576 031010410001
Package unit 5 Preps
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1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Mega prep
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 150−500 mL (high copy), 500−2000 mL (low copy)
Vector size < 300 kbp
Typical yield 500−2000 µg
Theoretical binding capacity 2000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 90−120 min/4−6 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Scope of delivery NucleoBond AX 2000 Columns, NucleoBond Folded Filters, buffers, RNase A
Gross weight (incl. packaging) 2356.06 g / 5.19 lbs
Packaging dimensions 215 x 215 x 165 mm / 8.46 x 8.46 x 6.50 Inch
Hazardous material Yes
Resuspension Buffer S1 Resuspension Buffer S1
Content 500 mL
REF 740516.1
Lysis Buffer S2 Lysis Buffer S2
Content 500 mL
REF 740517.1
Neutralization Buffer S3 Neutralization Buffer S3
Content 500 mL
REF 740518.1
Equilibration Buffer N2 Equilibration Buffer N2
Content 500 mL
REF 740527.1
Wash Buffer N3 Wash Buffer N3
Content 1000 mL
REF 740528.1
Elution Buffer N5 Elution Buffer N5
Content 500 mL
REF 740529.1