NucleoBond PC Prep 100 preparative scale purification endotoxin-free plasmid DNA

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740594 031010410001
Package unit 1 Preps
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Preparative scale isolation of endotoxin-free plasmid DNA more

Preparative scale isolation of endotoxin-free plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Preparative scale
Handling HPLC, FPLC
Lysate clarification Folded filter, sieving fabric
Automated use No
Sample material Bacteria, E. coli
Sample amount 5−20 L
Vector size < 300 kbp
Typical yield 80−100 mg
Theoretical binding capacity 100 mg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 20 h/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Scope of delivery NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Gross weight (incl. packaging) 10596.35 g / 23.36 lbs
Packaging dimensions 365 x 290 x 280 mm / 14.37 x 11.42 x 11.02 Inch
Hazardous material Yes