NucleoBond PC 10000 EF, Giga kit for endotoxin-free plasmid DNA

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Delivery time approx. 5 working days

740548 031010410001
Package unit 5 Preps
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NucleoBond PC 10000 EF is an anion exchange column kit for manual and semi automated use. The AX... more

NucleoBond PC 10000 EF is an anion exchange column kit for manual and semi automated use. The AX 10000 column contains our 1st generation anion exchange resin. The kit is capable of isolating high-purity, high-integrity plasmid DNA with endotoxin free quality (≤ 0.1 EU/µg DNA).

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Handling Gravity flow
Lysate clarification Bottle top filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 180 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A
Gross weight (incl. packaging) 12180 g / 26.85 lbs
Packaging dimensions 665 x 285 x 280 mm / 26.18 x 11.22 x 11.02 Inch
Hazardous material Yes
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