NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

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740420.50 031010413001
Package unit 50 Preps
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA more

2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Selling unit 10 Prep(s), 50 Prep(s)
Application Endotoxin-free plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Handling Gravity flow
Lysate clarification Column filter
Automated use Yes, can be automated on the Andrew+ pipetting robot
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size < 300 kbp
Typical yield 500 µg
Theoretical binding capacity 800 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 85 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Month(s)
Hazardous material Yes
Resuspension Buffer RES‑EF Resuspension Buffer RES‑EF
Content 1000 mL
REF 740386.1000
Lysis Buffer LYS‑EF Lysis Buffer LYS‑EF
Content 1000 mL
REF 740387.1000
Neutralization Buffer NEU‑EF Neutralization Buffer NEU‑EF
Content 1000 mL
REF 740388.1000
Equilibration Buffer EQU‑EF Equilibration Buffer EQU‑EF
Content 1000 mL
REF 740389.1000
Wash Buffer FIL‑EF Wash Buffer FIL‑EF
Content 600 mL
REF 740390.600
Wash Buffer WASH‑EF Wash Buffer WASH‑EF
Content 1000 mL
REF 740392.1000
Lyophilized RNase A Lyophilized RNase A
Content 100 mg
REF 740505
NucleoBond Xtra EF Buffer Set I NucleoBond Xtra EF Buffer Set I
Content 1 Set(s)
REF 740427