NucleoBond Xtra Midi EF, Midi kit for endotoxin-free plasmid DNA

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740420.50 031010413001
Content: 50 Preps
Quantity:
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA more

2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Application Endotoxin-free plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 10 Prep(s), 50 Prep(s)
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size < 300 kbp
Typical yield 500 µg
Theoretical binding capacity 800 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 85 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 18−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes
Resuspension Buffer RES‑EF Resuspension Buffer RES‑EF
Content 1000 mL
REF 740386.1000
$
Lysis Buffer LYS‑EF Lysis Buffer LYS‑EF
Content 1000 mL
REF 740387.1000
$
Neutralization Buffer NEU‑EF Neutralization Buffer NEU‑EF
Content 1000 mL
REF 740388.1000
$
Equilibration Buffer EQU‑EF Equilibration Buffer EQU‑EF
Content 1000 mL
REF 740389.1000
$
Wash Buffer FIL‑EF Wash Buffer FIL‑EF
Content 600 mL
REF 740390.600
$
Wash Buffer WASH‑EF Wash Buffer WASH‑EF
Content 1000 mL
REF 740392.1000
$
Lyophilized RNase A Lyophilized RNase A
Content 100 mg
REF 740505
$
NucleoBond Xtra EF Buffer Set I NucleoBond Xtra EF Buffer Set I
Content 1 Set(s)
REF 740427
$
Wash Buffer ENDO‑EF Wash Buffer ENDO‑EF
Content 1000 mL
REF 740391.1000
$
Elution Buffer ELU‑EF Elution Buffer ELU‑EF
Content 900 mL
REF 740393.900
$