NucleoBond PC 500 EF, Maxi kit for endotoxin-free plasmid DNA

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Delivery time approx. 5 working days

740550 031010410001
Package unit 10 Preps
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1st generation anion exchange technology for endotoxin-free plasmid DNA more

1st generation anion exchange technology for endotoxin-free plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 30−150 mL
Vector size < 300 kbp
Typical yield 100−500 µg
Theoretical binding capacity 500 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 100 min/2 preps
Typical downstream application Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Scope of delivery NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
Gross weight (incl. packaging) 3084.6 g / 6.8 lbs
Packaging dimensions 220 x 220 x 234 mm / 8.66 x 8.66 x 9.21 Inch
Hazardous material Yes
Resuspension Buffer S1-EF Resuspension Buffer S1-EF
Content 1000 mL
REF 740790.1
Lysis Buffer S2-EF Lysis Buffer S2-EF
Content 1000 mL
REF 740791.1
Neutralization Buffer S3-EF Neutralization Buffer S3-EF
Content 1000 mL
REF 740792.1
Equilibration Buffer N2-EF Equilibration Buffer N2-EF
Content 1000 mL
REF 740793.1
Wash Buffer N3-EF Wash Buffer N3-EF
Content 1000 mL
REF 740794.1
Wash Buffer N4-EF Wash Buffer N4-EF
Content 1000 mL
REF 740795.1
Elution Buffer N5-EF Elution Buffer N5-EF
Content 600 mL
REF 740796.1
Redissolving Buffer TE-EF Redissolving Buffer TE-EF
Content 1000 mL
REF 740797.1