NucleoSpin Plasmid Transfection-grade, Mini kit, MN

Preview: NucleoSpin Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

NucleoSpin Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

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Item number: 740490.50 031010411001

Package unit 50 Preps

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NucleoSpin Plasmid Transfection-grade is a spin column based kit for manual use. Our advanced... more

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NucleoSpin Plasmid Transfection-grade is a spin column based kit for manual use. Our advanced silica membrane based plasmid isolation kit is able to achieve endotoxin levels below ≤ 50 EU/µg plasmid DNA, which makes it viable for transfection of non-sensitive cells. Plasmid DNA is eluted in a slight alkaline buffer (5 mM Tris/HCl, pH 8.5), thus being ready for subsequent applications.

Application	Transfection-grade plasmid DNA isolation
Selling unit	10 Prep(s), 50 Prep(s), 250 Prep(s)
Target	Plasmid DNA
CE certified	No, research use only
Technology	Silica membrane technology and endotoxin reduction technology
Brand	NucleoSpin
Format	Mini prep
Handling	Centrifugation, vacuum
Lysate clarification	Centrifugation
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	1−5 mL
Vector size	< 25 kbp
Typical yield	15−30 µg
Theoretical binding capacity	35 µg
Typical purity A260/A280	1.8−1.85
Endotoxin level	≤ 50 EU/µg DNA
Elution volume	30−50 µL
Preparation time	20 min/6 preps
Typical downstream application	Transfection of sensitive cells and cell injections
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	27 Month(s)
Hazardous material	Yes