NucleoSpin Plasmid, Mini kit for plasmid DNA

Product configuration

Selling unit

Do you have any questions?
Please contact us.
Select variant first $0.00 * (0% Saved)

*taxes and shipping not included

740588.50 031010411001
Package unit 50 Preps
Rapid Mini preparation of plasmid DNA more

Rapid Mini preparation of plasmid DNA

Application High and low copy plasmid DNA purification
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL (high copy), 6−10 mL (low copy)
Vector size < 25 kbp
Typical yield 25−45 µg
Theoretical binding capacity 60 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level > 50 EU/µg DNA
Elution volume 50 µL
Preparation time 20 min/6 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 27 Month(s)
Hazardous material Yes
Resuspension Buffer A1 Resuspension Buffer A1
Content 1000 mL
REF 740911.1
Lysis Buffer A2 (without LyseControl) Lysis Buffer A2 (without LyseControl)
Content 1000 mL
REF 740912.1
Neutralization Buffer A3 Neutralization Buffer A3
Content 1000 mL
REF 740913.1
Wash Buffer AW Wash Buffer AW
Content 1000 mL
REF 740916.1
Elution Buffer AE Elution Buffer AE
Content 1000 mL
REF 740917.1
Wash Buffer AQ Concentrate Wash Buffer AQ Concentrate
Content 25 mL
REF 740995
NucleoSpin Plasmid Columns for plasmid DNA NucleoSpin Plasmid Columns for plasmid DNA
Content 50 Piece(s)
REF 740588.50S