NucleoSpin Plasmid Transfection-grade, Mini kit for ultrapure plasmid DNA

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740490.50 031010411001
Package unit 50 Preps
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A fast way to purify plasmids for transfection more

A fast way to purify plasmids for transfection

Application Transfection-grade plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology and endotoxin reduction technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp
Typical yield 15−30 µg
Theoretical binding capacity 35 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level < 50 EU/µg DNA
Elution volume 30−50 µL
Preparation time 20 min/6 preps
Typical downstream application Transfection of sensitive cells and cell injections
Storage temperature 18−25 °C
Shelf life (from production) 27 Months
Hazardous material Yes
Resuspension Buffer A1 Resuspension Buffer A1
Content 1000 mL
REF 740911.1
$
Lysis Buffer A2 (without LyseControl) Lysis Buffer A2 (without LyseControl)
Content 1000 mL
REF 740912.1
$
Neutralization Buffer A3 Neutralization Buffer A3
Content 1000 mL
REF 740913.1
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Wash Buffer AW Wash Buffer AW
Content 1000 mL
REF 740916.1
$
Elution Buffer AE Elution Buffer AE
Content 1000 mL
REF 740917.1
$
Wash Buffer AQ Concentrate Wash Buffer AQ Concentrate
Content 25 mL
REF 740995
$