NucleoSpin Plasmid EasyPure Mini kit for easy plasmid DNA purification

NucleoSpin Plasmid EasyPure, Mini kit for easy plasmid DNA purification

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Item number: 740727.50 031010411001

Package unit 50 Preps

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NucleoSpin Plasmid EasyPure is a spin column kit based for manual use. It has been developed for... more

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NucleoSpin Plasmid EasyPure is a spin column kit based for manual use. It has been developed for the rapid isolation of plasmid DNA from bacteria such as Escherichia coli. The innovative buffer design enables a one-step washing procedure, allowing isolation in under 10 minutes. Plasmid DNA is eluted in a slight alkaline buffer (5 mM Tris/HCl, pH 8.5), thus being ready-to-use for subsequent applications.

Application	High copy plasmid DNA purification
Selling unit	10 Prep(s), 50 Prep(s), 250 Prep(s)
Target	Plasmid DNA
CE certified	No, research use only
Technology	Silica membrane technology
Brand	NucleoSpin
Format	Mini prep
Handling	Centrifugation, vacuum
Lysate clarification	Centrifugation
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	1−5 mL
Vector size	< 25 kbp
Typical yield	15−30 µg
Theoretical binding capacity	35 µg
Typical purity A260/A280	1.8−1.85
Endotoxin level	> 50 EU/µg DNA
Elution volume	50 µL
Preparation time	14 min/6 preps
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transformation
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	27 Month(s)
Hazardous material	Yes