NucleoBond Xtra Midi Columns for transfection-grade plasmid DNA

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Delivery time approx. 5 working days

740410.10S 031010413001
Package unit 10 Piece(s)
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2nd generation of anion exchanger for fast purification of plasmid DNA more

2nd generation of anion exchanger for fast purification of plasmid DNA

Application Transfection-grade plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Handling Gravity flow
Lysate clarification Column filter
Automated use Yes, can be automated on the Andrew+ pipetting robot
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size < 300 kbp
Typical yield 500 µg
Theoretical binding capacity 1000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 70 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 72 Month(s)
Scope of delivery NucleoBond Xtra Midi Columns
Gross weight (incl. packaging) 274.55 g / 0.6 lbs
Packaging dimensions 235 x 162 x 77 mm / 9.25 x 6.38 x 3.03 Inch
Hazardous material No
Elution Buffer ELU Elution Buffer ELU
Content 600 mL
REF 740316.600
Equilibration Buffer EQU Equilibration Buffer EQU
Content 1000 mL
REF 740317.1000
Lysis Buffer LYS Lysis Buffer LYS
Content 1000 mL
REF 740329.1000
Neutralization Buffer NEU Neutralization Buffer NEU
Content 1000 mL
REF 740348.1000
Resupension Buffer RES Resupension Buffer RES
Content 1000 mL
REF 740363.1000
Wash Buffer WASH Wash Buffer WASH
Content 1000 mL
REF 740375.1000
NucleoBond Xtra Buffer Set I NucleoBond Xtra Buffer Set I
Content 1 Set(s)
REF 740417