NucleoBond PC 500, Maxi kit for transfection-grade plasmid DNA

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740574.50 031010410001
Package unit 50 Preps
1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Prep(s), 25 Prep(s), 50 Prep(s), 100 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 30−150 mL (high copy), 100−500 mL (low copy)
Vector size < 300 kbp
Typical yield 400−500 µg
Theoretical binding capacity 500 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 80−90 min/4 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transformation
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes
Resuspension Buffer S1 Resuspension Buffer S1
Content 500 mL
REF 740516.1
Lysis Buffer S2 Lysis Buffer S2
Content 500 mL
REF 740517.1
Neutralization Buffer S3 Neutralization Buffer S3
Content 500 mL
REF 740518.1
Equlibration Buffer N2 Equlibration Buffer N2
Content 500 mL
REF 740527.1
Wash Buffer N3 Wash Buffer N3
Content 1000 mL
REF 740528.1
Elution Buffer N5 Elution Buffer N5
Content 500 mL
REF 740529.1
NucleoBond Buffer Set I NucleoBond Buffer Set I
Content 1 Set(s)
REF 740601