NucleoBond PC 100, Midi kit for transfection-grade plasmid DNA

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740573.100 031010410001
Package unit 100 Preps
Quantity:
1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 20 Prep(s), 100 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 5−30 mL (high copy), 10−100 mL (low copy)
Vector size < 300 kbp
Typical yield 20−100 µg
Theoretical binding capacity 100 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 65 min/4−6 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 18−25 °C
Shelf life 39 Months
Hazardous material Yes
Resuspension Buffer S1 Resuspension Buffer S1
Content 500 mL
REF 740516.1
$
Lysis Buffer S2 Lysis Buffer S2
Content 500 mL
REF 740517.1
$
Neutralization Buffer S3 Neutralization Buffer S3
Content 500 mL
REF 740518.1
$
Equlibration Buffer N2 Equlibration Buffer N2
Content 500 mL
REF 740527.1
$
Wash Buffer N3 Wash Buffer N3
Content 1000 mL
REF 740528.1
$
Elution Buffer N5 Elution Buffer N5
Content 500 mL
REF 740529.1
$