NucleoBond PC 10000, Giga kit for transfection-grade plasmid DNA

$454.00 *

*taxes and shipping not included

Delivery time approx. 5 working days

740593 031010410001
Package unit 5 Preps
Quantity:
1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Package unit 5 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL (high copy), 1−4 L mL (low copy)
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 120−150 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 18−25 °C
Shelf life 39 Months
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Hazardous material Yes
Resuspension Buffer S1 Resuspension Buffer S1
Content 500 mL
REF 740516.1
$
Lysis Buffer S2 Lysis Buffer S2
Content 500 mL
REF 740517.1
$
Neutralization Buffer S3 Neutralization Buffer S3
Content 500 mL
REF 740518.1
$
Elution Buffer N5 Elution Buffer N5
Content 500 mL
REF 740529.1
$
NucleoBond Buffer Set I NucleoBond Buffer Set I
Content 1 Set(s)
REF 740601
$