NucleoSpin RNA, Mini kit for RNA purification
*taxes and shipping not included
Mini spin kit for isolation of RNA of highest integrity
Application | Isolation of RNA |
Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
Target | RNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation, vacuum |
Lysate clarification | Centrifugation, NucleoSpin Filter |
Automated use | No |
Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells |
Fragment size | > 200 nt |
Typical yield | 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells) |
Theoretical binding capacity | 200 µg |
Typical purity A260/A280 | 1.9–2.1 |
Typical RIN | > 9 |
Elution volume | 30–120 µL |
Preparation time | 35 min/6 preps |
Typical downstream application | RNA-Seq, RT−PCR |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Hazardous material | Yes |
swag_enterprise_search_suggestion_keywords | [{"input":"RNA","weight":5}] |
- Download Instruction NucleoSpin RNA
- Download Application Note - RNA isolation from bacteria and yeast
- Download Support protocol - Difficult to lyse tissue
- Download Support protocol - Disruption of RNAlater stabil. tissue with Mixer Mill MM300
- Download Support protocol - Saliva collected with Oragene RNA
- Download Support protocol - RNA from Paxgene tubes
- Download Tech Note - RNA isolation from insects
- Download RNA purification guide
- Download Leaflet NucleoSpin RNA
- Download Instruction NucleoSpin RNA (FR)
Reference list: NucleoSpin RNA – Isolation of RNA of highest quality and integrity
The NucleoSpin RNA is the ideal solution for the purification of high quality RNA from diverse sample materials. The purified RNA is ready to use for applications such as enzymatic labeling reactions (e.g., dye incorporation), reverse transcriptase-PCR (RT-PCR), RNA-sequencing, as well as for most other downstream applications. Only in the last two years, the NucleoSpin RNA kit has been cited in several hundred peer-reviewed publications. The following reference list shows some of the latest publications from 2023 and 2024 on NucleoSpin RNA listed by title and publication year of the study, journal and impact factor, as well as the downstream application.
Do you have any question? Feel free to contact our product specialists by phone:
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NucleoSpin RNA, Mini kit for RNA isolation: 740955.10/.50/.250
NucleoSpin RNA
Isolation of RNA of highest quality and integrity
The NucleoSpin RNA kit from MACHEREY‑NAGEL offers tailored and specialized solutions that will allow you to purify high quality RNA from a variety of sample materials.
In contrast to the RNeasy kit from QIAGEN, our NucleoSpin RNA kit includes rDNase and NucleoSpin filters. So there is no need for additional orders, as customers receive everything needed for a successful, DNA-free RNA purification with the MN kit. And it minimizes the risk of getting stuck mid-process due to lack of consumables.
The purified RNA allows downstream analysis in a variety of applications including enzymatic labeling reactions (e. g. dye incorporation), reverse transcriptase-PCR (RT-PCR) and RNA-sequencing.
Product workflow overview – 35 min/6 preps
Comparative data MN vs. QIAGEN
Matching quality, optimum value, reliable service
Our comparative study of NucleoSpin RNA and QIAGEN RNeasy demonstrates equivalent performance across key metrics (upper two panels, liver sample). Both kits yield high-quality RNA with comparable RIN values (9.2 vs. 9.6) and total yields (20.1 vs. 18.7 μg). Results of qRT-PCR show similar CT values for various genes across different sample types, indicating consistent RNA purity and integrity (lower two panels). For researchers seeking a costeffective alternative without compromising quality, NucleoSpin RNA offers equivalent performance to the market leader, with the added benefit of included rDNase and personalized support from our product specialists in the field. If you’re a current QIAGEN user, consider trying NucleoSpin RNA for your next RNA purification – you’ll experience comparable results with optimum convenience and customized service.
Appreciated by scientists worldwide
Our NucleoSpin RNA kits are highly appreciated by scientists due to their high quality, fast and easy workflows and our comprehensive service. Only in the last two years, the NucleoSpin RNA kit has been cited in several hundred peer-reviewed publications. The following reference list shows some of the latest publications from 2023 and 2024 on NucleoSpin RNA listed by title and publication year of the study, journal and impact factor, as well as the downstream application.
Request a free NucleoSpin RNA sample
Convince yourself of our proven NucleoSpin RNA Kit! Receive your test sample of 10 preparations. Get in touch with us and send us your sample inquiry.
Speak to an expert
Do you have any further questions, need technical advice or would like to request a quote? Please contact your local specialist at MACHEREY‑NAGEL!
Phone: 888-321-6224
Reference list