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NucleoSpin RNA, Mini kit for RNA purification
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Item number: 740955.50 031030430001
Package unit 50 Preps NucleoSpin RNA is a spin column based kit for manual use. It is intended for the isolation of... more
NucleoSpin RNA is a spin column based kit for manual use. It is intended for the isolation of high purity and integrity RNA from a wide range of sample materials like tissues, cultured cells, yeast and bacteria. Besides spin columns and buffers it contains rDNAse for on column DNA digestion.
Application | Isolation of RNA |
Selling unit | 4 Prep(s), 10 Prep(s), 50 Prep(s), 250 Prep(s) |
Target | RNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation, vacuum |
Lysate clarification | Centrifugation, NucleoSpin Filter |
Automated use | No |
Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells |
Fragment size | > 200 nt |
Typical yield | 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells) |
Theoretical binding capacity | 200 µg |
Typical purity A260/A280 | 1.9–2.1 |
Typical RIN | > 9 |
Elution volume | 30–120 µL |
Preparation time | 35 min/6 preps |
Typical downstream application | RNA-Seq, RT−PCR |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Hazardous material | Yes |
- Download Instruction NucleoSpin RNA
- Download Application Note - RNA isolation from bacteria and yeast
- Download Support protocol - RNA from difficult to lyse tissue
- Download Support protocol - Disruption of RNAlater stabil. tissue with Mixer Mill MM300
- Download Support protocol - Saliva collected with Oragene RNA (RE-100)
- Download Support protocol - RNA from Paxgene Blood RNA Tubes
- Download Tech Note - RNA isolation from insects
- Download RNA Purification Guide (EN)
- Download Leaflet NucleoSpin RNA
- Download Instruction NucleoSpin RNA (FR)
- Download RNA Purification Guide (ES)
Content 250 mL
REF 740400.250
$
Content 50 Preps
REF 740948.50
$
Content 50 Preps
REF 740966.50
$
Content 50 Preps
REF 740984.50
$