NucleoSpin DNA Yeast
*taxes and shipping not included
Isolation of total DNA from yeast and fungi – MN Bead Tubes Type C included
Application | Isolation of DNA |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation |
Automated use | No |
Sample material | Fungi, Yeast |
Sample amount | < 100 mg wet weight |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | Up to 20 µg (depending on sample amount and quality) |
Theoretical binding capacity | 60 µg |
Typical purity A260/A280 | 1.6–2.0 |
Elution volume | 100−200 µL |
Preparation time | 35 min/prep |
Typical downstream application | enzymatic reactions, PCR, qRT−PCR, Real-time PCR |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Hazardous material | Yes |
NucleoSpin DNA Yeast
- Optimized lysis with MN Bead Tubes Type C
- Up to 100 mg of sample input
- High genomic DNA yields
For DNA from yeast: Don’t lyse hard, lyse smart!
Protocols for isolation of DNA from yeast typically include either a chemical treatment to weaken the cell walls or a mechanical lysis with glass beads. The former requires additional hands-on time, the latter often is inefficient leading to prolonged lysis times and low DNA yields. The NucleoSpin DNA Yeast kit contains MN Bead Tubes Type C with corundum beads for most efficient lysis. Using corundum beads improves the lysis resulting in a drastically increased DNA yield. The kit delivers superior yields for various, popular yeast species, including Saccharomyces cerevisiae, Schizosachharomyces pombe and Pichia pastoris.
High DNA yields and efficient sample disruption from up to 100 mg sample
DNA was purified from 40–100 mg of cultured yeast (wet weight). DNA yield linearly increases with sample input.
Yeast samples (40 mg) were subjected to mechanical lysis with different types of beads. MN Bead Tubes Type C deliver the highest DNA yield in subsequent purification with NucleoSpin DNA Yeast kit.