NucleoSpin Blood XL, Maxi kit for DNA from blood
*taxes and shipping not included
Larce scale purification of high quality DNA from blood
Application | Isolation of DNA |
Selling unit | 10 Prep(s), 50 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Maxi prep |
Handling | Centrifugation |
Automated use | No |
Sample material | Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum |
Sample amount | 2–10 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 10⁸ cultured cells |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | 200–300 µg |
Typical concentration | 200–500 ng/µL |
Theoretical binding capacity | 700 µg |
Typical purity A260/A280 | 1.6–1.9 |
Elution volume | 600-2000 µL |
Preparation time | 60 min/prep |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 19 Month(s) |
Hazardous material | Yes |
NucleoSpin Blood XL
- Ready-to-use DNA in less than 60 min
- Complete removal of PCR inhibitors
- Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
- Consistently high DNA yields
NucleoSpin Blood XL procedure
Nucleospin Blood XL is designed for the rapid, large scale preparation of highly pure genomic DNA from up to 10 mL whole blood, serum, plasma, or other body fluids. Buffy coat volumes up to 2 mL can be processed easily. The obtained DNA can be used directly as template for PCR reactions or can be subjected to endonuclease digestion.
The binding capacity of the NucleoSpin Blood XL columns is about 700 μg of genomic DNA. The kit allows preparation of pure genomic DNA from 2–10 mL of human whole blood with an A260/A280 ratio between 1.7 and 1.9. Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20–30 μg per ml of healthy human blood. Blood treated either with EDTA, citrate, heparin, or CPDA can be used. Time for preparation of genomic DNA from blood with NucleoSpin Blood XL is approximately 1 hour.
Superior purification with the NucleoSpin Blood XL kit
DNA was isolated from 5ml of fresh blood samples. Results obtained showed isolation of highly pure genomic DNA. DNA Yields were in the expected range of sample specific variation.