NucleoSpin Blood L, Midi kit for DNA from blood
*taxes and shipping not included
Medium scale purification of high quality DNA from blood
Application | Isolation of DNA |
Selling unit | 20 Prep(s), 100 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Midi prep |
Handling | Centrifugation |
Automated use | No |
Sample material | Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum |
Sample amount | 0.2–2 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 2 x 10⁷ cultured cells |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | 40–60 µg |
Typical concentration | 200–500 ng/µL |
Theoretical binding capacity | 250 µg |
Typical purity A260/A280 | 1.6–1.9 |
Elution volume | 120-200 µL |
Preparation time | 60 min/prep |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Scope of delivery | NucleoSpin Blood L Columns, Collection Tubes, buffers, Proteinase K |
Gross weight (incl. packaging) | 652.05 g / 1.44 lbs |
Packaging dimensions | 230 x 160 x 102 mm / 9.06 x 6.30 x 4.02 Inch |
Hazardous material | Yes |
NucleoSpin Blood L
- Ready-to-use DNA in less than 60 min
- Complete removal of PCR inhibitors
- Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
- Consistently high DNA yields
NucleoSpin Blood L procedure
NucleoSpin Blood L is designed for the rapid, medium scale preparation of highly pure genomic DNA from up to 2 mL whole blood, serum, plasma, or other body fluids. Buffy coat up to 1 mL can be processed easily. If enrichment of leukocytes is not possible, for example after freezing of blood which leads to lysis of cells, large volumes of blood samples have to be processed. The obtained DNA can be used directly as template for PCR reactions or can be subjected to endonuclease digestion.
Due to the high binding capacity of the NucleoSpin Blood L Columns, which is about 250 μg of genomic DNA, the kit allows preparation of pure genomic DNA from up to 2 mL of human or animal whole blood with an A260/A280 ratio between 1.70 and 1.90. Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20–30 μg per ml of healthy human blood. Blood treated either with EDTA, citrate, heparin, or CPDA can be used. The procedure is optimized for a sample volume of 0.5–2 mL. Time for preparation of genomic DNA from blood with NucleoSpin Blood L is less than 1 hour..
Agarose gel analysis of the purified DNA from pooled blood samples.
DNA yield from 2 mL fresh or frozen pooled blood samples purified by NucleoSpin Blood L Vacuum
Highly reproducible results were obtained when using pooled blood samples, which demonstrates the robustness of the purification method. DNA was purified from identical aliquots of 2 mL fresh or frozen human blood. An average DNA yield of 68 μg with a CV of 9.5 % was obtained from fresh blood samples and an average yield of 33.9 μg with a CV of 12 % from frozen samples.
DNA integrity for fresh (left) and frozen (right) blood samples was investigated by agarose gel electrophoresis. 10 μL of the DNA purified from 2 mL pooled fresh and frozen blood samples was loaded on a 1 % TAE agarose gel (ethidium bromide stain). High molecular weight DNA combined with the absence of low molecular weight smear or degradation products demonstrate the integrity and quality of the purified gDNA.
Setup of the NucleoVac 96 Vacuum Manifold A maximum of 24 samples can be processed in parallel. Unused positions in the Column Holder Midi are closed with Dummy Columns.