NucleoSpin Blood L, Midi kit for DNA from blood

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740954.20 031040442001
Package unit 20 Preps
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NucleoSpin Blood L is a spin column based Midi kit for manual use. It is intended for the... more

NucleoSpin Blood L is a spin column based Midi kit for manual use. It is intended for the isolation of DNA from up to 2 mL whole blood, stabilized with EDTA, citrate, or heparin, serum, plasma, and other body fluids. Besides spin columns and buffers it contains Proteinae K for efficient blood lysis.

Application Isolation of DNA
Selling unit 20 Prep(s), 100 Prep(s)
Target DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Midi prep
Handling Centrifugation
Automated use No
Sample material Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum
Sample amount 0.2–2 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 2 x 10⁷ cultured cells
Fragment size 200 bp–approx. 50 kbp
Typical yield 40–60 µg
Typical concentration 200–500 ng/µL
Theoretical binding capacity 250 µg
Typical purity A260/A280 1.6–1.9
Elution volume 120-200 µL
Preparation time 60 min/prep
Typical downstream application enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 24 Month(s)
Scope of delivery NucleoSpin Blood L Columns, Collection Tubes, buffers, Proteinase K
Gross weight (incl. packaging) 652.05 g / 1.44 lbs
Packaging dimensions 230 x 160 x 102 mm / 9.06 x 6.30 x 4.02 Inch
Hazardous material Yes