NucleoSpin Blood L, Midi kit for DNA from blood using vacuum processing
*taxes and shipping not included
Delivery time approx. 5 working days
NucleoSpin Blood L Vacuum is a column based kit for the manual and automated isolation of genomic DNA from up to 2 mL blood samples using vacuum processing . It is intended for the isolation of DNA from blood samples stabilized with common anticoagulants. Besides medium sized columns it contains buffers and proteinase K.
Application | Isolation of DNA |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Midi prep |
Handling | Vacuum |
Automated use | No |
Sample material | Blood |
Sample amount | 1–2 mL |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | 50–80 µg (2 mL blood) |
Typical concentration | 100–130 ng/µL |
Theoretical binding capacity | 250 µg |
Typical purity A260/A280 | 1.6–1.9 |
Elution volume | 2 x 300 µL |
Preparation time | 75 min/24 preps |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Scope of delivery | NucleoSpin Blood L Columns, buffers, Proteinase K |
Gross weight (incl. packaging) | 892.4 g / 1.97 lbs |
Packaging dimensions | 233 x 161 x 136 mm / 9.17 x 6.34 x 5.35 Inch |
Hazardous material | Yes |

NucleoSpin Blood L Vacuum
- Up to 2 mL whole blood
- Ready-to-use DNA in 75 min
- Complete removal of PCR inhibitors
- Suitable for whole blood, treated with citrate, EDTA, heparin
Agarose gel analysis of the purified DNA from pooled blood samples.

DNA yield from 2 mL fresh or frozen pooled blood samples purified by NucleoSpin Blood L Vacuum
The NucleoSpin® Blood L Vacuum kit provides an optimal solution for manual, vacuum based DNA purification for 2 mL fresh and frozen blood samples. Convenient processing of up to 24 samples is possible using the Starter Set Midi in com bination with the NucleoVac 96 Vacuum Manifold. Blood samples are lysed using a Proteinase K incubation in the presence of a chaotropic salt-based buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded in two subsequent steps onto the binding columns. DNA is bound to the silica membrane as vacuum is applied to the column and buffers are filtered through. Impurities are removed from the bound DNA during several washing steps. Depending on the leukocyte count, typical yields up to 78 ug can be achieved. DNA purity (A260/280) is typically in the range of 1.7–1.9.

Highly reproducible results were obtained when using pooled blood samples, which demonstrates the robustness of the purification method. DNA was purified from identical aliquots of 2 mL fresh or frozen human blood. An average DNA yield of 68 μg with a CV of 9.5 % was obtained from fresh blood samples and an average yield of 33.9 μg with a CV of 12 % from frozen samples.
DNA integrity for fresh (left) and frozen (right) blood samples was investigated by agarose gel electrophoresis. 10 μL of the DNA purified from 2 mL pooled fresh and frozen blood samples was loaded on a 1 % TAE agarose gel (ethidium bromide stain). High molecular weight DNA combined with the absence of low molecular weight smear or degradation products demonstrate the integrity and quality of the purified gDNA.

Setup of the NucleoVac 96 Vacuum Manifold A maximum of 24 samples can be processed in parallel. Unused positions in the Column Holder Midi are closed with Dummy Columns.

Application Notes


