NucleoSpin Blood L, Midi kit for DNA from blood
*taxes and shipping not included
NucleoSpin Blood L is a spin column based Midi kit for manual use. It is intended for the isolation of DNA from up to 2 mL whole blood, stabilized with EDTA, citrate, or heparin, serum, plasma, and other body fluids. Besides spin columns and buffers it contains Proteinae K for efficient blood lysis.
Application | Isolation of DNA |
Selling unit | 2 Prep(s), 20 Prep(s), 100 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Midi prep |
Handling | Centrifugation |
Automated use | No |
Sample material | Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum |
Sample amount | 0.2–2 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 2 x 10⁷ cultured cells |
Fragment size | 200 bp–approx. 50 kbp |
Typical yield | 40–60 µg |
Typical concentration | 200–500 ng/µL |
Theoretical binding capacity | 250 µg |
Typical purity A260/A280 | 1.6–1.9 |
Elution volume | 120-200 µL |
Preparation time | 60 min/prep |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 24 Month(s) |
Scope of delivery | NucleoSpin Blood L Columns, Collection Tubes, buffers, Proteinase K |
Gross weight (incl. packaging) | 652.05 g / 1.44 lbs |
Packaging dimensions | 230 x 160 x 102 mm / 9.06 x 6.30 x 4.02 Inch |
Hazardous material | Yes |

NucleoSpin Blood L
- Ready-to-use DNA in less than 60 min
- Complete removal of PCR inhibitors
- Suitable for whole blood, treated with citrate, EDTA, heparin, CPDA
- Consistently high DNA yields
NucleoSpin Blood L procedure
NucleoSpin Blood L is designed for the rapid, medium scale preparation of highly pure genomic DNA from up to 2 mL whole blood, serum, plasma, or other body fluids. Buffy coat up to 1 mL can be processed easily. If enrichment of leukocytes is not possible, for example after freezing of blood which leads to lysis of cells, large volumes of blood samples have to be processed. The obtained DNA can be used directly as template for PCR reactions or can be subjected to endonuclease digestion.
Due to the high binding capacity of the NucleoSpin Blood L Columns, which is about 250 μg of genomic DNA, the kit allows preparation of pure genomic DNA from up to 2 mL of human or animal whole blood with an A260/A280 ratio between 1.70 and 1.90. Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20–30 μg per ml of healthy human blood. Blood treated either with EDTA, citrate, heparin, or CPDA can be used. The procedure is optimized for a sample volume of 0.5–2 mL. Time for preparation of genomic DNA from blood with NucleoSpin Blood L is less than 1 hour.
