NucleoSpin 96 Plasmid, 96‑well kit for plasmid DNA

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740625.4 03101041200111
Package unit 384 Preps
Rapid Mini preparation of plasmid DNA in proven 96‑well format more

Rapid Mini preparation of plasmid DNA in proven 96‑well format

Application High copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format 96-well plate
Package unit 96 Prep(s), 384 Prep(s), 2304 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification NucleoSpin Plasmid Filter Plate
Automated use Yes
Application note available with Hamilton, Tecan
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp
Typical yield 4−30 µg
Theoretical binding capacity 20 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level > 50 EU/µg DNA
Elution volume 75−150 µL
Preparation time 45 min/plate
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transformation
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Hazardous material Yes
Buffer A4 Concentrate Buffer A4 Concentrate
Content 200 mL
REF 740914.1
Resuspension Buffer A1 Resuspension Buffer A1
Content 1000 mL
REF 740911.1
Lysis Buffer A2 (without LyseControl) Lysis Buffer A2 (without LyseControl)
Content 1000 mL
REF 740912.1
Neutralization Buffer A3 Neutralization Buffer A3
Content 1000 mL
REF 740913.1
Wash Buffer AW Wash Buffer AW
Content 1000 mL
REF 740916.1
Elution Buffer AE Elution Buffer AE
Content 1000 mL
REF 740917.1