NucleoMag kit, clean up & size selection of NGS library prep reactions

Preview: NucleoMag kit for clean up and size selection of NGS library prep reactions

NucleoMag kit for clean up and size selection of NGS library prep reactions

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Item number: 744970.50 031020423001

Package unit 50 mL

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The NucleoMag NGS Clean-up and Size Select kit allows for the tailored preparation of NGS... more

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The NucleoMag NGS Clean-up and Size Select kit allows for the tailored preparation of NGS libraries using simple clean-up, single or double sided size selection protocols. The kit allows a precise fine-tuning of your sizes selection with an accuracy of 50 bp. It is possible to select DNA with a chosen size threshold or within a specific size-range. The beads are also applicable for the simple nucleic acid clean-up from reaction mixtures, such as cDNA synthesis or in-vitro transcription reactions.

Application	Clean up and size selection for NGS library preps
Selling unit	5 mL, 50 mL, 500 mL
Target	Clean up
CE certified	No, research use only
Technology	Magnetic bead technology
Brand	NucleoMag
Format	Magnetic beads
Handling	Magnetic separation
Automated use	Yes
Sample material	Reaction mixtures from NGS library kits
Sample amount	7.5 pg−5 µg nucleic acids in NGS reaction mixtures
Fragment size	150 bp−approx. 800 bp
Typical recovery	≥ 80 %
Elution volume	10−100 µL
Preparation time	40−120 min/96 preps
Typical downstream application	Next Generation Sequencing
Storage temperature	2–8 °C / 36–46 °F
Shelf life (from production)	24 Month(s)
Hazardous material	No

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744970.50_en-EN_GHS.pdf

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Product	ng/μL	A260 / A280	A260 / A230
MN Beads	14.4 ± 2.1	2.3 ± 0.1	2.5 ± 0.7
RNAClean* XP	14.4 ± 1.6	2.3 ± 0.1	2.2 ± 0.8

Product	ng/μL	A260 / A280	A260 / A230
MN Beads	14.4 ± 2.1	2.3 ± 0.1	2.5 ± 0.7
RNAClean* XP	14.4 ± 1.6	2.3 ± 0.1	2.2 ± 0.8

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Technology	Magnetic beads
Applications	Size selection for next-generation-sequencing workflows PCR Clean-up RNA or cDNA Clean-up after purifications or enzymatic reactions (i. e. IVT or DNA digestion)
Recovery	70 – 100 %
Elution volume	10 – 100 μL
Shelf-life (from production)	24 months
Product sizes	5 mL, 50 mL, 500 mL

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NucleoMag kit for clean up and size selection of NGS library prep reactions

Magnetic bead-based DNA and RNA Clean-up

NucleoMag NGS Clean-up and Size Select

Product at a glance

Schematic Workflow Overview

One Clean-up kit
for all samples!

fghfghgf

Use your
current protocol.
No changes
needed.

One Clean-up kit
for all samples!

fghfghgf

Use your
current protocol.
No changes
needed.

Application data – DNA Clean-Up and Size Selection

Size selection of fragment mix

Reliable reproducibility in DNA clean-up procedures

Fragment size analysis of prepared NGS libraries

Sequencing analysis

Cited in more than 50 publications

Application data – RNA Clean-Up

RNA Clean-Up with reliable recovery rates as well as high purities

High-integrity RNA after Clean-up with NucleoMag NGS Clean-Up and Size Select kit

NucleoMag NGS Clean-Up and Size Select kit is suitable for Clean-ups of RNA transcripts after in vitro transcription reactions

Technical data & contact information

Magnetic bead-based DNA and RNA Clean-up

NucleoMag NGS Clean-up and Size Select

Product at a glance

Schematic Workflow Overview

One Clean-up kitfor all samples!

fghfghgf

Use yourcurrent protocol.No changesneeded.

One Clean-up kitfor all samples!

fghfghgf

Use yourcurrent protocol.No changesneeded.

Application data – DNA Clean-Up and Size Selection

Size selection of fragment mix

Reliable reproducibility in DNA clean-up procedures

Fragment size analysis of prepared NGS libraries

Sequencing analysis

Cited in more than 50 publications

Application data – RNA Clean-Up

RNA Clean-Up with reliable recovery rates as well as high purities

High-integrity RNA after Clean-up with NucleoMag NGS Clean-Up and Size Select kit

NucleoMag NGS Clean-Up and Size Select kit is suitable for Clean-ups of RNA transcripts after in vitro transcription reactions

Technical data & contact information

One Clean-up kit
for all samples!

Use your
current protocol.
No changes
needed.

One Clean-up kit
for all samples!

Use your
current protocol.
No changes
needed.