MN Bead Tubes Type B
*taxes and shipping not included
Delivery time approx. 5 working days
2 mL tubes with 40–400 µm glass beads; for homogenization of Gram-positive and Gram-negative bacteria
| Application | Sample homogenization |
| Target | Accessories |
| Format | Mini prep |
| CE certified | No, research use only |
| Handling | Bead beating |
| Scope of delivery | MN Bead Tubes Type B |
| Storage temperature | 15–25 °C / 59–77 °F |
| Gross weight (incl. packaging) | 172.95 g / 0.38 lbs |
| Packaging dimensions | 151 x 109 x 83 mm / 5.94 x 4.29 x 3.27 Inch |
| Hazardous material | No |
| Shelf life (from production) | 72 Month(s) |
MN Bead Tube Type B
Cultured microorganisms such as bacteria are complex samples that often require mechanical homogenization to ensure a complete lysis. Our MN Bead Tubes Type B include glass beads in a mixture of sizes (40 - 400 um) and are an efficient tool for effective homogenization of cultured bacterial samples.
Scalable options
Solutions for small sample amounts
Bulk solutions for different sample amounts
HTP solution
- 2ml screw cap tubes
- Prefilled with 40-400 µm glass beads
- compatible with common bead beating devices
- click here for more information
- bulk beads
- 40-70µm glass beads
- recommended to be mixed in a ratio of 1:5 with B2 bulk glass beads
- compatible with common bead beating devices
- click here for more information
- Pack of prefilled tube strips (8x12)
- Prefilled with 40-400µm glass beads
- compatible with common plate disruption device
- click here for more information
Sample processing recommendations
MN Bead Tube Holder in combination with a Vortex-Genie 2
Sample processing recommendation using a RETSCH Mixer Mill
| Sample type | Processing time | Buffer and volume | Recommended kit |
|---|---|---|---|
| Microbial samples | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin Microbial DNA |
| Sample type | Processing time | Buffer and volume | Bead Tube | Recommended kit |
|---|---|---|---|---|
| Gram negative bacteria (Escherichia Coli, Vibro fischeri | 4-6 min, 30Hz | <40mg microbial pellet + 140µl IME, 10µl Proteinase K, 2.5µl RNaseA | MN Bead Tubes Type B | NucleoSpin Microbial DNA |
| Gram positive bacteria (Bacillus subtilis, Corynebacterium glutamicum) | 12 min, 30Hz | <40mg microbial pellet + 140µl IME, 10µl Proteinase K, 2.5µl RNaseA | MN Bead Tubes Type B | NucleoSpin Microbial DNA |
| Sample type | Processing time | Buffer and volume | Bead tube | Recommended kit |
|---|---|---|---|---|
| Gram negative bacteria (Escherichia coli, Vibrio fischeri) | 2x4 min at 30 Hz | <40mg microbial pellet + 140µl IME, 10µl Proteinase K, 2.5µL RNase A | MN 96 Bead Plate Type B | NucleoSpin Microbial DNA |
| Gram positive bacteria (Bacillus subtilis, Corynebacterium glutamicum) | 6-12min at 30Hz | <40mg microbial pellet + 140µl IME, 10µl Proteinase K, 2.5µL RNase A | MN 96 Bead Plate Type B | NucleoSpin Microbial DNA |
MN Bead Tubes Type B
MN 96 Bead Plate Type B
MN Bead Tubes (with the exception of MN Bead Tubes Type F) can be combined with most disruption devices. Please find below the recommendation for MN Bead Tubes Type B in combination with different purification kits and the RETSCH Mixer Mill. Click here to find our latest Application Note for MN Bead Tubes and the RETSCH Mixer Mill.
In order to obtain optimal DNA yields from sample materials, a complete disruption of the sample is necessary. Sample disruption efficiency depends on several parameters such as the type of bead tube or plate, time and frequency of disruption, and also the disruption device.
Please see below for our suggested recommendations for implementing the MN Bead Tubes Type B with various nucleic acid purification kits:
Sample disruption using the MN Bead Tube Holder is a very straight forward and easy method to homogenize your sample. The MN Bead Tube Holder is available for 2 ml and 5 ml bead tubes.
- bulk beads
- 30 - 400µm glass beads
- recommended to be mixed in a ratio of 5:1 with B1 bulk glass beads
- compatible with common bead beating devices
- click here for more information
Compatible with the following kits
