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NucleoSpin Plasmid, Mini kit for plasmid DNA
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Item number: 740588.50 031010411001
Package unit 50 Preps Rapid Mini preparation of plasmid DNA more
Rapid Mini preparation of plasmid DNA
Application | High and low copy plasmid DNA purification |
Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Handling | Centrifugation, vacuum |
Lysate clarification | Centrifugation |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | 1−5 mL (high copy), 6−10 mL (low copy) |
Vector size | < 25 kbp |
Typical yield | 25−45 µg |
Theoretical binding capacity | 60 µg |
Typical purity A260/A280 | 1.8−1.85 |
Endotoxin level | > 50 EU/µg DNA |
Elution volume | 50 µL |
Preparation time | 20 min/6 preps |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 27 Month(s) |
Hazardous material | Yes |
- Download Instruction NucleoSpin Plasmid (NoLid)
- Download Support protocol - M13 DNA
- Download Support protocol - Agrobacterium tumefaciens
- Download Support protocol - Plasmid DNA from yeast
- Download Leaflet
- Download Instruction NucleoSpin Plasmid (FR)
- Download Supplementary protocol for isolation of high-copy plasmid DNA (vacuum)