NucleoSpin 96 Blood, 96-well kit for DNA from blood
*taxes and shipping not included
Versatile purification of high quality DNA from whole blood in proven 96‑well plate format
Application | Isolation of DNA |
Selling unit | 96 Prep(s), 384 Prep(s), 2304 Prep(s) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | 96-well plate |
Handling | Centrifugation, vacuum |
Automated use | Yes |
Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
Sample material | Blood, Cells |
Sample amount | 200 µL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), 2 x 10⁶ cultured cells |
Fragment size | 300 bp–approx. 50 kbp |
Typical yield | 4–6 µg |
Theoretical binding capacity | 20 µg |
Typical purity A260/A280 | 1.8–1.9 |
Elution volume | 100 µL |
Preparation time | 70 min/plate |
Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
Storage temperature | 15–25 °C / 59–77 °F |
Shelf life (from production) | 19 Month(s) |
Hazardous material | Yes |
- Download Instruction NucleoSpin 96 Blood
- Download Application Note - Uppsala biobanking on Hamilton Genomic STARlet
- Download Application Note - Eppendorf epMotion 5075
- Download Application Note - Tecan Freedom EVO
- Download Application Note - Hamilton Genomic STARlet
- Download Application Note - Hamilton MICROLAB STAR
- Download Leaflet
NucleoSpin96 Blood
- Time-saving parallel isolation of genomic DNA from whole blood
- Optimized for manual or automated use under vacuum
- Improved flow-rate reduces risk of clogging – no cross-contamination,
reliable results - Innovative MN Wash Plate minimizes risk of cross-contamination
- Complete processing possible at room temperature
- Consistently high yields
NucleoSpin96 Blood automated use with the epMotion 5075
NucleoSpin96 Blood is designed for the parallel isolation of genomic DNA from whole blood. After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum NucleoSpin96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.
Yield and purity of DNA - Reproducibility of DNA purification
gDNA was isolated from a pool of human blood samples stored at 4 °C. 200 µL of human blood were used for DNA purification. DNA yield and purity were determined by UV measurement