NucleoProtect VET Reagent, DNA / RNA stabilization and sample inactivation
*taxes and shipping not included
The NucleoProtect VET reagent has been developed for the stabilization, transportation and inactivation of infectious veterinary specimens for molecular diagnostic purposes. The reliable preservation of DNA / RNA makes cold chain logistics obsolete and increases reliability of molecular test results; Infectious viruses are immediately inactivated. Bottled NucleoProtect VET reagent for filling into transport or strorage vessels.
| Application | Inactivation of viral pathogens in animal whole blood or swab samples and preservation of nucleic acids for molecular diagnostics |
| Technology | DNA / RNA stabilization and inactivation reagent |
| Sample material | Animal whole blood or swab samples |
| Sample storage | -20 - 37 °C |
| Target | Viral RNA and DNA |
| Format | Bottled NucleoProtect VET reagent |
| CE certified | No, not intended for human use |
| Selling unit | 50 mL, 500 mL |
| Hazardous material | Yes |
| Shelf life (from production) | 24 Month(s) |
NucleoProtect VET
DNA / RNA stabilization and sample inactivation for molecular testing
Convenient sampling, inactivation of infectious viruses, storage, and shipment, all made easy with the NucleoProtect VET stabilization and inactivation solution.
Molecular results will be unaffected regardless of whether your veterinary specimens are shipped cold or at ambient temperatures, require multiple days for shipment, or are frozen for longer term storage. Stabilizing your samples means bringing higher accuracy and less variance into your results. The intuitive design including peel-off barcodes allows easy sampling on farms or in the wild.
Automated and manual purification
NucleoMag DNA Microbiome can be processed manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
Efficient isolation of DNA from fecal samples
Fecal samples from different animals (rabbit, sheep, chicken, rat) were subjected to a mechanical lysis procedure with MN Bead Tubes Type A. DNA was purified from the homogenates using the NucleoMag DNA Microbiome kit (MN) and a competitor kit (Q) according to the manufacturers instructions. DNA yield and purity were measured photometrically. DNA eluates were used in a qPCR for the bacterial 16s rRNA gene. DNA eluates obtained with NucleoMag DNA Microbiome show a superior purity for all fecal sample types. The yield is comparable (sheep) or better (rabbit, chicken, rat) than with the competitor kit. The qPCR performance is comparable (rabbit, sheep, rat) or better (chicken) than with the competitor kit.
Superior DNA yield and purity from various soil types
Two different lysis buffer options plus optional addition of the chemical additive Enhancer SX allow fine tuning for maximum yield and purity.
High quality DNA from human and animal stool
Superior DNA yield and purity from various soil types
Two different lysis buffer options plus optional addition of the chemical additive Enhancer SX allow fine tuning for maximum yield and purity.
Efficient lysis system – even suitable for difficult-to-lyse microorganisms
Total DNA from 400 mg cropping soil was purified with NucleoSpin Soil using Lysis Buffer SL2 in combination with Enhancer SX. 2 μl of undiluted eluates were analyzed in PCR using, order specific primer systems.
Click to read more:
NucleoMag DNA/RNA water
The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.
Automated and manual purification of DNA and RNA from filters
Compatibility with different filtration systems
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).
Safe and easy sampling
NucleoProtect VET products are intuitive, single-step storage and transport systems for collection of swab or blood specimens.
Reliable test results
The reagent removes virulence while reliably preserving nucleic acids in the sample. Sample stabilization increases accuracy and precision of molecular tests, reduces variability, and brings standardization and comparability between laboratories to a new level.
Simplifying logistics
The nucleic acid stabilizer makes cold chain logistics obsolete and allows shipment and storage at ambient temperatures.
User protection
Effective inactivation of infectious viruses reduces the risk of laboratory-acquired infections and spread of diseases.
Time saving
NucleoProtect VET samples can be transferred directly to pre-analytical nucleic acid extraction procedures which streamlines diagnostic testing and laboratory routines.
Secure traceability
Both swab and blood tubes come with double barcode labels with peel-off section for definitive sample tracking and management (including human readable text).
Secure traceability
Both swab and blood tubes come with double barcode labels with peel-off section for definitive sample tracking and management (including human readable text).
Scalable options
Solutions for small sample amounts
Principle of method
NucleoProtect VET consists of a novel, non-hazardous proprietary formula that destroys viral envelopes and denatures capsid proteins, removing virulence from swab or blood samples whilst maintaining the integrity of nucleic acids. Inactivation occurs within 30 minutes at ambient temperature (15-25°C). No further processing of the sample is required ahead of nucleic acid extraction.
Scalable options
Solutions for small sample amounts
| NucleoProtect VET Blood Tube | |
|---|---|
| Format | Blood collection tube pre-filled with 4.0 mL NucleoProtect VET reagent, |
| Sample material | 1.6 mL animal whole blood (ruminants, swine, poultry, equine, companion animals,...) |
| Package unit | Box of 50 tubes |
| Tube diameter | ⌀ 13 mm |
| Barcode format | Double barcode with peel-off section, Code 128 with embedded human readable text for definitive sample identification and simplified sample management |
| Cannulas | Compatible with different sizes of standard Luer cannulas (cannulas not included) |
| Reference number | REF 740755 |
Sampling formats
The NucleoProtect VET reagent is available in three different sampling formats:
- Pre-filled syringes for blood collection: NucleoProtect VET Blood Tubes
- Pre-filled swab collection tubes: NucleoProtect VET Swab Tubes
- Bottled reagent: NucleoProtect VET reagent
Scalable options
Solutions for small sample amounts
| NucleoProtect VET Swab Tube | |
|---|---|
| Format | Screw cap swab collection tube pre-filled with 1.5 mL NucleoProtect® VET reagent |
| Sample material | Animal swab samples such as oral, throat, nasal or pharyngeal swabs |
| Package unit | Box of 50 tubes, including 50 sterile, individually packed flocked swabs |
| Tube diameter | ⌀ 13 mm |
| Barcode format | Double barcode with peel-off section, Code 128 with embedded human readable text for definitive sample identification and simplified sample management |
| Swab length | 150 mm flocked swab (predetermined breaking point at 80 mm) |
| Reference number | REF 740760 |
Scalable options
Solutions for small sample amounts
| NucleoProtect VET Reagent | |
|---|---|
| Format | Inactivation and DNA / RNA stabilization reagent for veterinary samples |
| Sample material | Animal whole blood or swab samples |
| Application | Bulk solution of NucleoProtect VET reagent for filling into transport or storage vessels |
| Package unit | 50 mL, 500 mL |
| Reference number | REF 740750.50 / 740750.500 |
NucleoMag DNA Microbiome - Application notes
NucleoMag DNA Microbiome can be automated on many liquid handling plaforms. Please find below an overview of application notes:
TAN Bead application note
Automated processing of soil, stool and biofilm
NucleoMag DNA Microbiome epMotion 5075t//5073t
In the application note we show that the NucleoMag DNA Microbiome kit in combination with the epMotion 5073t or 5075t quickly and reliably purifies high-quality host and pathogenic genomic DNA from human stool samples. We also show superior inhibitor removal and efficient lysis with enhancer SX for soil samples demonstrating the purification of high quality DNA from soil microbial communities.
Automated purification of DNA from soil and human stool samples on the Eppendorf epMotion 5075t/5073t
Secure sample tracking by double barcode labels with peel-off section. The peel-off part is self-adhesive and can be transferred to sample request forms.
Barcode: Code 128 with embedded human readable text.
The integrated swab holder, designed to facilate the capture of the swab handle, allows both simple and safe sample collection in the field and efficient sample processing in the laboratory. The swab can be easily removed from the sample by unscrewing the cap.
The integrated swab holder, designed to facilate the capture of the swab handle, allows both simple and safe sample collection in the field and efficient sample processing in the laboratory. The swab can be easily removed from the sample by unscrewing the cap.
The product design allows gentle collection of animal blood samples according to the aspiration technique; the blood samples are immediately mixed and stabilized with the prefilled NucleoProtect VET inactivation reagent. The blood collection tubes are compatible with all common Luer cannulas.
Handling features
NucleoMag DNA/RNA water
The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.
Automated and manual purification of DNA and RNA from filters
Compatibility with different filtration systems
NucleoProtect VET reagent inactivates > 99.99 % of pathogenic animal viruses, independent of the background matrix
Infectious viruses are immediately inactivated within the NucleoProtect VET solution, enabling safe handling for both veterinarians and for laboratory staff. The inactivation capacity of the NucleoProtect VET reagent has been demonstrated for various background matrices and viruses which are known to have different envelope structures as well as susceptibilities to disinfectants: FMDV (small non-enveloped virus), BTV-5 (large non-enveloped virus), LSDV (large enveloped virus), PPRV and BCoV (enveloped virus). The table shows the level of virus titer reduction as shown via the Tissue Culture Infections Dose (TCID) assay (incubation time: 30 min).
These data were collected in a joint collaboration project with the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).
Proven inactivation of high risk viruses
NucleoMag DNA/RNA water
The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.
Automated and manual purification of DNA and RNA from filters
Compatibility with different filtration systems
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).
Reliable DNA and RNA preservation for molecular testing
NucleoProtect VET stabilization capacity was tested with all naturally occurring viral genome structures, such as ssRNA, dsRNA, and dsDNA. Nucleic acids are reliably preserved over different storage periods and conditions, making cold chain logistics obsolete and reducing the risk of sample degradation.
NucleoMag DNA/RNA water
The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.
Automated and manual purification of DNA and RNA from filters
Compatibility with different filtration systems
NucleoProtect VET Swab Tubes increase reproducibility and accuracy of molecular diagnostic testing
Bovine saliva was spiked with lumpy skin disease virus (LSDV). Foam swabs, rayon swabs, and flocked swabs were dipped into the saliva sample for 30 seconds each (n= 4). From each swab type, two swabs were placed into NucleoProtect Swab tubes and two were placed into screw cap tubes containing 1.5 mL of cell culture medium. The tubes were stored at room temperature, 4 °C and 37 °C respectively for the indicated time period (T0 = 2 h RT). LSDV DNA was extracted in duplicates from each tube using the NucleoMag VET Kit and analyzed via qPCR. The data show 4 Cq-values (blue / grey dots) and the corresponding mean value(orange bar). The results show the stabilization of LSDV DNA in NucleoProtect Swab tubes at the different storage conditions and the high accuracy of detection of LSDV with different swab types. In contrast, the sensitivity of the detection of LSDV in unstabilized swab samples clearly decreases after storage at room temperature or 37 °C, independent of swab type. NucleoProtect VET Swab tubes can be used with the included swab (flocked swab) or with the swab of your choice.
These data were collected in a joint collaboration project with the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).
Application data - NucleoProtect VET Swab Tubes
In-vivo experiments show higher sensitivity for NucleoProtect VET-stabilized oral swab samples from FMDV positive cattle
Five foot-and-mouth disease virus (FMDV) positive cattle were sampled using standard rayon swabs. The swab samples were subsequently incubated in 1.5 mL NucleoProtect VET stabilization solution or minimal essential medium for 24 h at ambient temperature and then purified in duplicates using the MACHEREY‑NAGEL NucleoMag VET kit. FMDV RNA was detected using qRT-PCR. Data show the mean value of duplicate samples and the max and min values. The comparison of the mean Cq-values of swab samples stored in NucleoProtect VET stabilization solution versus minimal essential medium demonstrates the increase in sensitivity, as FMDV is detected between 1.2 and 4.3 Cq-values earlier in the NucleoProtect VET stabilization solution. Note: Samples were collected from individual cattle, resulting in variable viral titers and Cq-values.
These data were collected in a joint collaboration project with the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
NucleoMag DNA/RNA water
The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.
Automated and manual purification of DNA and RNA from filters
Compatibility with different filtration systems
NucleoProtect VET Blood Tubes reliably preserve nucleic acids of various pathogenic viruses independent of viral titer concentration and animal species
Bovine, goat, sheep, and porcine blood samples were spiked with low, medium and high virus titers of the canine distemper virus (ss(-)RNA), porcine enterovirus (ss(+)RNA), bluetongue virus (dsRNA), or the vaccinia virus (dsDNA). Spiked blood samples were collected in NucleoProtect VET Blood Tubes and stored according to the indicated storage conditions (T0= 2 h at room temperature). Nucleic acids were extracted in triplicate per test time point using the NucleoMag VET kit and subsequently analyzed via qPCR. The data show 12 Cq-values for each test time point (triplicate extraction for each of the four animal blood types (blue dots) and the resulting mean value (orange bar)). The data indicate that nucleic acids are efficiently preserved independent of animal blood type, virus species or virus titer. The overall Cq variance is below ± 1 Cq on average.
Compatibility with different filtration systems
A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.
NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).
Application data - NucleoProtect VET Blood Tubes
NucleoProtect VET Blood Tubes reduce variability and increase reliability of veterinary test results
Bovine, sheep, goat and porcine blood samples were spiked with low and high viral titers of the bluetongue virus (BTV-8). Samples were either collected with NucleoProtect VET Blood tubes or as EDTA-blood samples. Samples were shipped to four independent laboratories which extracted the nucleic acids within a time period of 2 – 7 days post collection using the MACHEREY-NAGEL NucleoMag VET kit (extraction was performed in duplicates). All eluates were analyzed in duplicates in one laboratory using a qRT-PCR assay with 45 cycles. The data represent 16 resulting Cq-values (blue dots) and the resulting mean value (orange bar). The data show that with EDTA blood collection tubes, the variation in analytical results can be very large, and even false negative results and high losses in sensitivity are possible. In contrast, data for the NucleoProtect VET Blood tubes show highly reproducible results and minimal variance, regardless of animal species, shipping and storage conditions.
These data were collected in a joint collaboration project with the Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health of Germany.
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NucleoProtect VET Blood Tube, DNA / RNA stabilization and sample inactivation 
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