NucleoBond HMW DNA, high molecular weight DNA from diverse samples

Preview: NucleoBond HMW DNA, high molecular weight DNA from diverse sample materials

NucleoBond HMW DNA, high molecular weight DNA from diverse sample materials

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Item number: 740160.20 031040454001

Package unit 20 Preps

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The combination of gentle enzymatic lysis and DNA purification using anion exchange... more

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The combination of gentle enzymatic lysis and DNA purification using anion exchange chromatography in the NucleoBond HMW DNA kit provide minimal DNA shearing and contaminations for optimal, pure, high molecular weight (HMW) DNA. The extracted HMW DNA is ideal for long-range single-molecule sequencing.

Application	Isolation of DNA
Selling unit	2 Prep(s), 20 Prep(s)
Target	DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	Midi prep
Handling	Gravity flow
Automated use	No
Sample material	Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast
Sample amount	< 300 mg human/animal tissue, < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample
Fragment size	2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis)
Typical yield	Depending on amount and quality of sample
Theoretical binding capacity	600 µg
Typical purity A260/A280	1.8–1.9
Elution volume	50–250 µL
Preparation time	2 h/12 preps (incl. 30 min lysis)
Typical downstream application	Long-read sequencing, Next Generation Sequencing
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	39 Month(s)
Hazardous material	Yes