NucleoBond AX 500, Maxi columns kit for transfection-grade plasmid DNA

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740531.50 031010410001
Package unit 50 Piece(s)
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1st generation anion exchanger for purification of plasmid DNA more

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Piece(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 30−150 mL (high copy), 100−500 mL (low copy)
Vector size < 300 kbp
Typical yield 400−500 µg
Theoretical binding capacity 500 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 80−90 min/4 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing
Storage temperature 18−25 °C
Shelf life (from production) 72 Months
Hazardous material No