NucleoBond 96 Xtra EF, 96‑well kit for endotoxin-free plasmid DNA

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740430.4 031010414001
Package unit 384 Preps
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA more

2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Application Endotoxin-free plasmid DNA isolation
Selling unit 96 Prep(s), 384 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format 96-well plate
Handling Gravity flow , vacuum for filtration and precipitation
Lysate clarification NucleoBond Filter Plate
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp, < 300 kbp (without the NucleoBond Finalizer Plate)
Typical yield 2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes)
Theoretical binding capacity 50 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Plate
Elution volume 100−200 µL
Preparation time 120 min/plate
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Month(s)
Hazardous material Yes
Resuspension Buffer RES‑EF Resuspension Buffer RES‑EF
Content 1000 mL
REF 740386.1000
Lysis Buffer LYS‑EF Lysis Buffer LYS‑EF
Content 1000 mL
REF 740387.1000
Neutralization Buffer NEU‑EF Neutralization Buffer NEU‑EF
Content 1000 mL
REF 740388.1000
Equilibration Buffer EQU‑EF Equilibration Buffer EQU‑EF
Content 1000 mL
REF 740389.1000
Wash Buffer FIL‑EF Wash Buffer FIL‑EF
Content 600 mL
REF 740390.600
Wash Buffer WASH‑EF Wash Buffer WASH‑EF
Content 1000 mL
REF 740392.1000
Lyophilized RNase A Lyophilized RNase A
Content 100 mg
REF 740505
NucleoBond Xtra EF Buffer Set I NucleoBond Xtra EF Buffer Set I
Content 1 Set(s)
REF 740427