NucleoBond 96 Xtra EF, 96-well kit for endotoxin-free plasmid DNA

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740430.4 031010414001
Package unit 384 Preps
2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA more

2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Application Endotoxin-free plasmid DNA isolation
Selling unit 96 Prep(s), 384 Prep(s)
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format 96-well plate
Handling Gravity flow , vacuum for filtration and precipitation
Lysate clarification NucleoBond Filter Plate
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp, < 300 kbp (without the NucleoBond Finalizer Plate)
Typical yield 2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes)
Theoretical binding capacity 50 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Plate
Elution volume 100−200 µL
Preparation time 120 min/plate
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Hazardous material Yes
Resuspension Buffer RES-EF Resuspension Buffer RES-EF
Content 1000 mL
REF 740386.1000
Lysis Buffer LYS-EF Lysis Buffer LYS-EF
Content 1000 mL
REF 740387.1000
Neutralization Buffer NEU-EF Neutralization Buffer NEU-EF
Content 1000 mL
REF 740388.1000
Equilibration Buffer EQU-EF Equilibration Buffer EQU-EF
Content 1000 mL
REF 740389.1000
Wash Buffer FIL-EF Wash Buffer FIL-EF
Content 600 mL
REF 740390.600
Wash Buffer WASH-EF Wash Buffer WASH-EF
Content 1000 mL
REF 740392.1000
Lyophilized RNase A Lyophilized RNase A
Content 100 mg
REF 740505
NucleoBond Xtra EF Buffer Set I NucleoBond Xtra EF Buffer Set I
Content 1 Set(s)
REF 740427
Wash Buffer ENDO-EF Wash Buffer ENDO-EF
Content 1000 mL
REF 740391.1000
Elution Buffer ELU-EF Elution Buffer ELU-EF
Content 900 mL
REF 740393.900