MN Beads Type D (bulk), DNA and nuclease free
*taxes and shipping not included
Delivery time approx. 5 working days
3 mm steel beads; for homogenization of insects, crustaceans, lipid rich tissue, DNA and nuclease free
| Application | Sample homogenization |
| Target | Accessories |
| Format | Bulk |
| CE certified | No, research use only |
| Handling | Bead beating |
| Scope of delivery | Bulk beads |
| Storage temperature | 15–25 °C / 59–77 °F |
| Gross weight (incl. packaging) | 543.6 g / 1.2 lbs |
| Packaging dimensions | 53 x 53 x 104 mm / 2.09 x 2.09 x 4.09 Inch |
| Hazardous material | No |
| Shelf life (from production) | 72 Month(s) |
MN Bead Tube Type D
Homogenization of difficult to lyse tissues or even insects or microbes within tissues is a challenging lyis approach when purifying nucleic acids. The MN Bead Tubes Type D are 3mm steel beads that are able to overcome these challenges.
Scalable options
Solutions for small sample amounts
HTP solution
- 2ml screw cap tubes
- Prefilled with 3 mm steel beads
- compatible with common bead beating devices
- Click here for more product information
- Pack of prefilled tube strips (8x12)
- Prefilled with 3 mm steel beads
- compatible with common plate disruption device
- Click here for more product information
Sample processing recommendations
MN Bead Tube Holder in combination with a Vortex-Genie 2
Sample processing recommendation using a RETSCH Mixer Mill
| Sample type | Processing time | Buffer and volume | Recommended kit |
|---|---|---|---|
| Brain tissue (fresh or frozen) | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Adipose tissue (fresh or frozen) | Approx. 20 min, full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Fatty fish tissue (fresh or frozen) | Approx. 20 min, full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Insect material (Drosophila melanogaster) | Approx. 0.5-15 min, full speed, RT | according to the standard protocol | NucleoSpin DNA Insect |
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
|---|---|---|---|---|
| Soft tissue (brain, liver, adipose or fatty fish tissue) | 1 min at 10Hz followed by 10s at 20Hz | <40mg tissue + 40µl LBP + 100µl IME + 10µL ProK + 2.5µL RNase A | MN Beads Tube Type D | NucleoMag DNA Bacteria |
| Insects (fresh, frozen, ethanol preserved) | 0.5-10 min at 30Hz | Insect + 100µL BE + 40µL MG, 10µL Proteinase K | MN Beads Tube Type D | NucleoSpin DNA Insect |
| Lipid rich tissue | 0.5-10 min at 30Hz | according to the standard protocol | MN Beads Tube Type D | NucleoSpin DNA Lipid Tissue |
| Filamentous fungi (Aspergillus nidulans, melon mold, citrus mold, Potato mold) | 1-2 min at 25Hz | <40mg cell pellet+ 100µl BE+ 40µl MG + 10µL Proteinase | MN Beads Tube Type D | NucleoMag DNA Bacteria |
| Sample type | Processing time | Buffer and volume (supplementary) | Bead tube | Recommended kit |
|---|---|---|---|---|
| Soft tissue (brain, liver, adipose or fatty fish tissue) | 1 min at 10 Hz followed by 10sec at 20Hz | < 40mg tissue + 40µl LBP+ 100µl IME + 10µL Proteinase K + 2.5µl RNase A | MN 96 Bead Plate Type D | NucleoMag DNA Bacteria |
| Filamentous fungi ( Aspergillus nidulans, Meldon mold, citrus mold, potato mold) | 2x1 min at 20 Hz | <40mg microbial pellet + 100µl BE + 40µl MG + 10µl Proteinase K | MN 96 Bead Plate Type D | NucleoMag DNA Bacteria |
MN Bead Tubes Type D
MN 96 Bead Plate D
An alternative in sample disruption of biological materials are bead beating mills. MN Bead Tubes (with the exception of MN Bead Tubes Type F) can be combined with most disruption devices. Please find below the recommendation for MN Bead Tubes D incombination with different purification kits and the RETSCH Mixer Mill. Click here to find our latest Application Note for MN Bead Tubes and the RETSCH Mixer Mill.
In order to obtain optimal yields of DNA from sample material, a complete disruption of the sample material is necessary. Sample disruption efficiency depends on the parameters such as type of bead tube or bead plate, time and frequency and also the discrution device. Please find below our suggested recommendations for the MN Bead Tubes Type D in combination with different nucleic acid purifiaction kits.
Sample disruption using the MN Bead Tube Holder is a very straight forward and easy method to homogenize your sample. The MN Bead Tube Holder is available for 2 ml and 5 ml bead tubes.
Compatible with the following kits
Bulk solutions for different sample amounts
- bulk beads
- DNA and nuclease free
- 3 mm steel beads
- compatible with common bead beating devices
- click here for more information
