EC HPLC column (analytical), NUCLEOSIL CHIRAL-3, 5 µm, 250x4 mm

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720350.40 02402027803015
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Silica-based phase for HPLC enantiomer separation. Principle interaction modes - charge-transfer... more

Silica-based phase for HPLC enantiomer separation. Principle interaction modes - charge-transfer interactions, hydrogen bonds, dipole-dipole interactions and steric effects. For control of the optical purity of a substance, the two columns NUCLEOSIL CHIRAL-2 and NUCLEOSIL CHIRAL-3 allow to select conditions such that the minor enantiomer, which is present as an impurity, is eluted before the main peak. Thus, overlapping peaks are avoided. This makes an exact quantification of the impurity much easier. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL CHIRAL-3
USP listing L36
Surface chemistry N-(3,5-dinitrobenzoyl)-L-phenylglycine (chiral selector) modification
Mode Special application or diverse
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column n-heptane – 2-propanol – TFAA (trifluoroacetic acid) (100/0.05/0.05, v/v/v)
Recommended application(s) Chiral, enantiomers, Control in chiral organic syntheses, Diastereomers, Enantiomers, HPLC, Pesticides, Pharmaceuticals, Propionic acid derived herbicides, Stereoisomers
Endcapped No
Carbon content Not available or proprietary
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 400 bar / 5800 psi
Column volume 3.15 mL
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 293.6 g / 0.65 lbs
Packaging dimensions 115 x 400 x 50 mm / 4.53 x 15.75 x 1.97 Inch
Hazardous material No