EC HPLC column (analytical), NUCLEOSIL CHIRAL-1, 5 µm, 250x4 mm

EC HPLC column (analytical), NUCLEOSIL CHIRAL-1, 5 µm, 250x4 mm
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720081.40 02402027803005
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Silica-based phase for HPLC enantiomer separation. Principal interaction mode - formation of... more

Silica-based phase for HPLC enantiomer separation. Principal interaction mode - formation of ternary mixed-ligand complexes with Cu(II) ions. Differences in the stability of the diastereomeric complexes cause chromatographic separation. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL CHIRAL-1
USP listing L32
Surface chemistry L-hydroxyproline – Cu²⁺ complexes (chiral selector) modification
Mode Special application or diverse
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column 0.5 mmol/L copper sulfate solution
Recommended application(s) Chiral, enantiomers, Enantiomers with two polar functional groups, HPLC, Lactic acid, α-amino acids, α-hydroxycarboxylic acids
Endcapped No
Carbon content Not available or proprietary
pH stability 2.0–8.0
Pore size 120 Å
Specific surface according to BET 200 m²/g
Pore volume 0.65 mL/g
Temperature stability (max) 60 °C
Pressure stability (max) 400 bar
Column volume 3.15 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis