EC HPLC column (analytical), NUCLEOSIL 500-5 C18 PPN, 5 µm, 250x4 mm

EC HPLC column (analytical), NUCLEOSIL 500-5 C18 PPN, 5 µm, 250x4 mm
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Delivery time approx. 5 working days

720258.40 0240503050
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Special reversed phase with polymerically bonded alkyl chains with exclusively hydrophobic... more

Special reversed phase with polymerically bonded alkyl chains with exclusively hydrophobic interactions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Dynamic protein binding capacity per g packing - 22 mg BSA, 40 mg cytochrome C. Especially suited for large peptides and medium-size hydrophilic proteins.

Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 500-5 C18 PPN
USP listing L1
Surface chemistry Special octadecyl (C18, ODS) modification
Mode Special application or diverse
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Methanol
Recommended application(s) Biological macromolecules, HPLC, Large peptides, Medium-size hydrophilic proteins
Endcapped No
Carbon content Not available or proprietary
pH stability 1.0–9.0
Pore size 500 Å
Specific surface according to BET 35 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 250 bar / 3625 psi
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 238.77 g / 0.52 lbs
Packaging dimensions 405 x 117 x 54 mm / 15.94 x 4.61 x 2.13 Inch
Hazardous material No