EC HPLC column (analytical), NUCLEOSIL 100-5 NH2-RP, 5 µm, 250x4.6 mm

EC HPLC column (analytical), NUCLEOSIL 100-5 NH2-RP, 5 µm, 250x4.6 mm
$655.00 *

*taxes and shipping not included

Delivery time approx. 5 working days

720095.46RP 024020272042
Package unit 1 Piece(s)
Quantity:
Besides cyano modifications, amino modifications belong to the most frequently used polar silica... more

Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions. These HPLC columns are packed with polar aminopropyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-5 NH2-RP
USP listing L8
Surface chemistry Aminopropyl (NH2) modification
Mode Multi-mode (RP and NP), Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
Endcapped No
Carbon content 3.5 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Pore volume 1 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 400 bar
Column volume 4.15 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis