EC HPLC column (analytical), NUCLEOSIL 100-5 CN, 5 µm, 250x4.6 mm

EC HPLC column (analytical), NUCLEOSIL 100-5 CN, 5 µm, 250x4.6 mm
$655.00 *

*taxes and shipping not included

Delivery time approx. 5 working days

720090.46 024020272036
Package unit 1 Piece(s)
Quantity:
The cyanopropyl phase is able to generate a clearly recognizable different retention behavior... more

The cyanopropyl phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. The polarity can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, π-π, and also hydrophobic interactions. Therefore, CN phases show a distinct selectivity for polar organic compounds as well as for molecules containing π-electron systems (e.g., analytes with double bonds). This HPLC phase can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. This HPLC column is packed with CN modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-5 CN
USP listing L10
Surface chemistry Cyano, nitrile (CN) modification
Mode Multi-mode (NP and RP), Normal phase (NP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column n-heptane
Recommended application(s) HPLC, Organic acids, Reversed phase and normal phase chromatography, Steroids, Tricyclic antidepressants
Endcapped No
Carbon content 5.0 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Pore volume 1 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 400 bar
Column volume 4.15 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis