EC HPLC column (analytical), NUCLEOSIL 100-5 C6H5, 5 µm, 250x4.6 mm

EC HPLC column (analytical), NUCLEOSIL 100-5 C6H5, 5 µm, 250x4.6 mm
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720956.46 024020272034
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Relatively nonpolar phase for RP and ion pairing chromatography. Polarity similar to C8, but... more

Relatively nonpolar phase for RP and ion pairing chromatography. Polarity similar to C8, but with different selectivity for polycyclic aromatic hydrocarbons, polar aromatics, fatty acids etc. Separation mechanism based on π–π interactions and hydrophobic (van der Waals) interactions, some residual silanol interactions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-5 C6H5
USP listing L11
Surface chemistry Phenyl (C6H5) modification
Mode Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) Fatty acids, HPLC, PAH, Polar aromatics, Polarity similar to C8, but with different selectivity, Polycyclic aromatic hydrocarbons, Relatively nonpolar phase for RP and ion pairing chromatography
Endcapped No
Carbon content 8.0 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Pore volume 1 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 400 bar
Column volume 4.15 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis