EC HPLC column (analytical), NUCLEOSIL 100-5 C18 MPN, 5 µm, 250x4 mm

EC HPLC column (analytical), NUCLEOSIL 100-5 C18 MPN, 5 µm, 250x4 mm
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Delivery time approx. 5 working days

720231.40 0240503050
Package unit 1 Piece(s)
Quantity:
Special reversed C18 (ODS) phase with monomerically bonded alkyl chains, brush type structure.... more

Special reversed C18 (ODS) phase with monomerically bonded alkyl chains, brush type structure. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Predominantly hydrophobic forces with a small portion of hydrophilic interactions. The dynamic protein binding capacity per g packing - 6 mg BSA, 110 mg cytochrome C. Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-5 C18 MPN
USP listing L1
Surface chemistry Special octadecyl (C18, ODS) modification
Mode Special application or diverse
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Methanol
Recommended application(s) Biological macromolecules, HPLC
Endcapped No
Carbon content Not available or proprietary
pH stability 1.0–9.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Pore volume 1 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 250 bar
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis