EC HPLC column (analytical), NUCLEOSIL 100-10 NH2, 10 µm, 250x4.6 mm

EC HPLC column (analytical), NUCLEOSIL 100-10 NH2, 10 µm, 250x4.6 mm
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720025.46 024020272042
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Besides cyano modifications, amino modifications belong to the most frequently used polar silica... more

Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions. These HPLC columns are packed with polar aminopropyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-10 NH2
USP listing L8
Surface chemistry Aminopropyl (NH2) modification
Mode Multi-mode (NP and RP), Normal phase (NP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 10 µm
Eluent in HPLC column n-heptane
Recommended application(s) HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
Endcapped No
Carbon content 3.5 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 400 bar / 5800 psi
Column volume 4.15 mL
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 271.9 g / 0.6 lbs
Packaging dimensions 115 x 400 x 50 mm / 4.53 x 15.75 x 1.97 Inch
Hazardous material No