EC HPLC column (analytical), NUCLEODUR HILIC, 5 µm, 250x4.6 mm

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760550.46 02402027007005
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Separation science is always looking for new and effective strategies to accomplish the tasks of... more

Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.

Method HPLC, analytical
Brand NUCLEODUR
Base material NUCLEODUR - high purity silica
Phase NUCLEODUR HILIC
USP listing Not available or proprietary
Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification
Mode Hydrophilic Interaction Chromatography (HILIC)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4.6 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) Amino acids, Highly polar analytes, HILIC applications HPLC, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins
Endcapped No
Carbon content 7.0 %
pH stability 2.0–8.5
Pore size 110 Å
Specific surface according to BET 340 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 600 bar / 8700 psi
Column volume 4.15 mL
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 231.67 g / 0.51 lbs
Packaging dimensions 405 x 117 x 54 mm / 15.94 x 4.61 x 2.13 Inch
Hazardous material No