Veterinary pathogen surveillance via water and air sampling technologies

Several airborne viruses pose risk to swine, poultry, cattle, or veterinary workers. Airborne viruses may be transmitted through the air via aerosols, especially when animals are kept in the confined spaces of agricultural facilities. Such aerosols can be concentrated with air sampling devices or filters and analyzed for airborne pathogens. Sampling for pathogens in livestock production facilities or in process water is a state of the art veterinary surveillance technology for easy and early detection of lifestock hazards. 

MACHEREY-NAGEL has developed various solutions and protocols for the purification of viral and bacterial nucleic acids from filters, water, and environmental samples, ensuring maximum flexibility and purity even with difficult, highly contaminated, and inhibitor-containing samples:

• Single spin- and magnetic bead-based options for manual or automated processing
• Automation-friendly components: Contact us for automation support!
• Suited for air- and waterborne bacterial and viral disease monitoring via filtering/concentration technology
• Indoor or outdoor monitoring
• Pathogen monitoring in drinking water, agricultural process water, or production water for fish and crustaceans
• Verified methods for sample concentration

NucleoMag DNA/RNA Water

The NucleoMag DNA/RNA Water enables manual or automated extraction of either DNA, RNA, or both molecules in parallel from filter-concentrated samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate detection of viruses or microbes (depending on concentration method) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology, air pathogen surveillance, and for estimation of process water quality through determination of the microbial/viral load of the sample.

Automated and manual purification of DNA and RNA from filters

NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many magnetic rod systems and liquid handling platforms.

Compatibility with different filtration systems

NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).

Compatibility with different filtration systems

A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.

Various water samples can be processed with NucleoMag DNA/RNA Water.

DNA and RNA can be extracted from a range of water samples, spanning from turbid to clear, as well as from air filters.

Efficient detection of microbes from water and air samples

Various water samples and an air sample were filtered, and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag DNA / RNA Water kit.

NucleoSpin Soil for filtered samples

Our NucleoSpin Soil kit is well suited for DNA extraction from filter samples. A combination of mechanical lysis with MN Bead Tubes Type A and the right lysis buffer chemistry allows reliable isolation of high-quality DNA even from hard to lyse microorganisms present in water. The superior NucleoSpin Soil inhibitor removal technology eliminates PCR Inhibitors like humic acids. Purified DNA is suitable for PCR and other downstream applications.

NucleoSpin Soil - DNA purification from water samples

Please find more detailed information and protocol recommendations in our Tech Note.

Efficient DNA isolation from water filters

Efficient DNA isolation from water filters with NucleoSpin Soil

A qPCR was performed with nucleic acids isolated from round filters demonstrating reliable results across different filtration systems

Methods for sample concentration and microbiome studies

Please click on links below for more information and method protocols for sample concentration of virus particles prior to nucleic acid purification. These methods are used by researchers and government agencies in routine wastewater-based epidemiology (WBE) for COVID-19 and reflect an effective and low-cost solution to track disease outbreaks:

• Virus concentration with Ceres Nanotrap Magnetic Virus Particles
• Sample concentration on the INNOVAPREP concentrating Pipette Select
• Sample concentration via ultrafiltration (.i.e. Centricon tubes)
• Sample concentration via precipitation (e.g. PEG)
• Concentration via electronegative membranes

Please click here for more information on nucleic acid isolation for microbiome studies.

Automated sample extraction made easy!

Are you planning to automate your sample preparation? The automation specialists at MACHEREY-NAGEL can support you with implementing the NucleoMag DNA/RNA Water kit on your robotic platform. Contact us for automation support or for free, ready-to-go scripts, e.g. for the Thermo KingFisher or other magnetic rod and liquid handler platforms.

E-Mail: bio-tech@mn-net.com I Tel.: +49 24 21 969 270