SARS-CoV-2 extraction from wastewater concentrated with Nanotrap Magnetic Virus Particles

NucleoSpin or NucleoMag nucleic acid purification kits provide a successfull combination for waster water surveillance. Read more about the NucleoSpin recommendation and suggestions in combination with Nanotrap Magnetic Virus Particles.

For low throughput in combination with Nanotrap Magnetic Virus Particles we recommend to use the 

• NucleoSpin RNA Stool
• NucleoSpin RNA Virus

If you have further questions please contact our Tech Support Team.

The combination of the Ceres Nanotrap beads for wastewater concentration and the MACHEREY-NAGEL NucleoMag DNA/RNA Water kit for viral RNA extraction is an excellent option for wastewater surveillance labs looking for a low or high throughput workflow method, and it is an especially attractive method for labs wanting to automate the workflow.

• Reliable performance with excellent removal of PCR inhibitors

• Workflow is automatable on a Kingfisher Flex or Apex platform with a 24-well head. 

For more information click here to read our Technote.

Protocol overview

Application data

Limit of Detection (LoD) Range-Finding Study

Heat inactivated SARS-CoV-2 was spiked into wastewater that tested negative for SARS-CoV-2 at concentration ranges of 0–10,000 copies/mL (A) and subsequently 0–100 copies/mL (B). Samples were processed using Ceres Nanosciences’ Nanotrap particles with the MACHEREY-NAGEL NucleoMag DNA/RNA Water kit on the Kingfisher Apex. Viral detection was achieved using the 2019-nCoV CDC EUA assay (A) or the Promega SARS-CoV-2 Wastewater RT-qPCR Kit (B) with a N1 primer/probe system. LoD was determined when 95 % of all biological replicates returned positive values. N=3 (A) and n=20 (B). LoD was determined to be 50 copies/mL.

Detection of SARS-CoV-2 in wastewater samples in four geographic regions of the United States

10 mL of wastewater was concentrated using Ceres Nanosciences’ Nanotrap particles, and viral RNA was extracted using the MACHEREY-NAGEL NucleoMag DNA/RNA Water kit on the KingFisher Apex. Viral detection was achieved using the Promega SARS-CoV-2 Wastewater RT-qPCR Kit for both N1 and N2 primers. Samples were from four geographic areas: Seattle, WA (1–9), San Bernardino, CA (10–15), Storrs, CT (16–19), or Los Angeles, CA (20–26). Detection as Ct is seen in (A). In (B), the amount of virus in each sample was quantified and normalized to a BCoV spike-in control.

Comparison of viral concentration methods: HA Filter vs. Nanotrap particles

10 mL of wastewater was processed using either Ceres Nanosciences’ Nanotrap particles or HA filtration. Viral RNA was then extracted and purified from all samples using the MACHEREY-NAGEL NucleoMag DNA/RNA Water kit on the KingFisher Apex. Viral detection was achieved using the Promega SARS-CoV-2 Wastewater RT-qPCR Kit for both N1 and N2 primers. Samples were from two wastewater treatment plants in Seattle, WA (A, B) and another in Storrs, CT (C).