Sample concentration on the INNOVAPREP Concentrating Pipette Select
Convenient and reliable nucleic acid extraction from wastewater samples!
Combine INNOVAPREP’s convenient sample concentration device CP-Select™ with MACHEREY-NAGEL’s proven nucleic acid purification procedures for reliable nucleic acid concentration and purification from wastewater samples with the NucleoMag DNA/RNA Water , NucleoSpin RNA Stool and NucleoSpin RNA Virus.
- Reliable performance
- Excellent removal of PCR inhibitors
- Convenient sample concentration without the need for time consuming and labor-intensive methods.
Application data
NucleoSpin RNA Virus allows reliable, inhibition-free qPCR detection of MS2 phage RNA from wastewater concentrates.
RNA was purified from wastewater concentrates spiked with different amounts of MS2 phage RNA using the NucleoSpin RNA Virus kit. qRT-PCR analysis was performed with a Taqman probe for MS2 RNA using the SensiFastTM Probe One-Step Lo-ROX kit from Bioline on an Applied Biosystems 7500 Real-Time PCR system. MS2 bacteriophage RNA was detected consistently and reliably.
NucleoMag DNA/RNA Water allows reliable, inhibition-free qPCR detection of MS2 phage RNA from wastewater concentrates.
MS2 bacteriophage RNA was spiked into wastewater concentrates from 20 or 40 ml or into water in two different concentrations. RNA was isolated using the NucleoMag DNA/RNA Water kit. qRT-PCR analysis was performed with a Taqman probe for MS2 RNA using the SensiFastTM Probe One-Step Lo-ROX kit from Bioline on an Applied Biosystems 7500 Real-Time PCR System.
Superior RNA and DNA recovery from wastewater concentrates
NucleoMag DNA/RNA Water was used to isolate DNA and RNA from wastewater concentrates spiked with different amounts of T7 DNA (upper data) or MS2 phage RNA (lower data). The recovered DNA and RNA were compared to the respective DNA and RNA input demonstrating superior DNA and RNA recovery from wastewater concentrates.
Detection of armored control RNA in wastewater concentrates.
Different amounts of armored control RNA were spiked into wastewater concentrates from 40 ml sample before RNA isolation. qRT-PCR results demonstrate the reliable detection of different amounts of SARS-CoV-2 RNA purified with NucleoMag DNA/RNA Water.