Precipitation of wastewater samples

Concentration of viral particles is essential for efficient nucleic acid isolation from water samples. Explore our recommendations about how to combine the PEG precipitation method for wastewater concentration with our NucleoMag DNA/RNA Water kit for nucleic acid extraction. Read more in the paragraphs below about our recommendations how to combine our kits with ultrafiltration and precipitation. Please Click here to read the technical note.

Precipitation – Step by Step protocol recommendation for NucleoMag DNA/RNA Water

The following step by step protocol describes the procedure for a 40 mL wastewater sample. The precipitation method can be adapted to larger volumes.

Application data

Detection of MS2 bacteriophage RNA in wastewater precipitates

40 ml wastewater samples were precipitated using PEG/ NaCl. MS2 bacteriophage RNA was spiked into the resuspended precipitates in a dilution series (n = 2 for each dilution) and isolated using the NucleoMag DNA/RNA Water kit (see step-by-step protocol). qRT-PCR analysis was performed with a Taqman probe for MS2 RNA using the SensiFastTM Probe One-Step Lo-ROX kit from Bioline on an Applied Biosystems 7500 Real-Time PCR System. MS2 bacteriophage RNA was detected consistently and reliably over a range of a dilution series with excellent linearity (R² = 0.9995).