NucleoBond PC 2000 EF, Mega kit for endotoxin-free plasmid DNA
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NucleoBond PC 20000 EF is an anion exchange column kit for manual use. The AX 2000 column contains our 1st generation anion exchange resin. The kit is capable of isolating high-purity, high-integrity plasmid DNA with endotoxin-free quality (≤ 0.1 EU/µg DNA), rendering it ideal for transfection of sensitive cells. Depending on the sample input, yields of up to 2 mg are achievable.
| Application | High and low copy plasmid DNA purification |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Endotoxin level | < 0.1 EU/µg DNA |
| Format | Mega prep |
| Gross weight (incl. packaging) | 3985.1 g / 8.79 lbs |
| Handling | Gravity flow |
| Hazardous material | Yes |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi |
| Lysate clarification | Bottle top filter |
| Packaging dimensions | 280 x 245 x 310 mm / 11.02 x 9.65 x 12.20 Inch |
| Preparation time | 150 min/2 preps |
| Sample amount | 150−500 mL |
| Sample material | Bacteria, E. coli |
| Scope of delivery | NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A |
| Shelf life (from production) | 27 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 2000 µg |
| Typical downstream application | Automated or manual sequencing, Cloning, Hybridization, In-vitro transcription, PCR, Transfection |
| Typical purity A260/A280 | 1.8−1.95 |
| Typical yield | 500−2000 µg |
| Vector size | < 300 kbp |
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