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NucleoBond PC 500 EF, Maxi kit for endotoxin-free plasmid DNA

1st generation anion exchange technology for endotoxin-free plasmid DNA

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level < 0.1 EU/µg DNA
Format Maxi prep
Gross weight (incl. packaging) 3084.6 g / 6.8 lbs
Handling Gravity flow
Hazardous material Yes
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Lysate clarification Folded filter
Packaging dimensions 220 x 220 x 234 mm / 8.66 x 8.66 x 9.21 Inch
Preparation time 100 min/2 preps
Sample amount 30−150 mL
Sample material Bacteria, E. coli
Scope of delivery NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
Shelf life (from production) 27 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 500 µg
Typical downstream application Automated or manual sequencing, Cloning, Hybridization, In-vitro transcription, PCR, Transfection
Typical purity A260/A280 1.8−1.95
Typical yield 100−500 µg
Vector size < 300 kbp

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