NucleoBond PC Prep 100 preparative scale purification endotoxin-free plasmid DNA

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Item number: 740594

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Preparative scale isolation of endotoxin-free plasmid DNA

Application	High and low copy plasmid DNA purification
Automated use	No
Brand	NucleoBond
CE certified	No, research use only
Endotoxin level	< 0.1 EU/µg DNA
Format	Preparative scale
Gross weight (incl. packaging)	10596.35 g / 23.36 lbs
Handling	HPLC, FPLC
Hazardous material	Yes
Isopropanol precipitation	Centrifugation
Lysate clarification	Folded filter, sieving fabric
Packaging dimensions	365 x 290 x 280 mm / 14.37 x 11.42 x 11.02 Inch
Preparation time	20 h/prep
Sample amount	5−20 L
Sample material	Bacteria, E. coli
Scope of delivery	NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Shelf life (from production)	39 Month(s)
Storage temperature	15–25 °C / 59–77 °F
Target	Plasmid DNA
Technology	Anion exchange chromatography
Theoretical binding capacity	100 mg
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Typical purity A260/A280	1.8−1.95
Typical yield	80−100 mg
Vector size	< 300 kbp

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Instruction NucleoBond PC EF

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