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NucleoBond PC 500, Maxi kit for transfection-grade plasmid DNA

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level 1−10 EU/µg DNA
Format Maxi prep
Handling Gravity flow
Hazardous material Yes
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Lysate clarification Folded filter
Preparation time 80−90 min/4 preps
Sample amount 30−150 mL (high copy), 100−500 mL (low copy)
Sample material Bacteria, E. coli
Selling unit 2 Prep(s), 10 Prep(s), 25 Prep(s), 50 Prep(s), 100 Prep(s)
Shelf life (from production) 39 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 500 µg
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transformation
Typical purity A260/A280 1.8−1.95
Typical yield 400−500 µg
Vector size < 300 kbp

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