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NucleoSpin Plasmid (NoLid) for QIAcube

Rapid Mini preparation of plasmid DNA on QIAcube

Application High and low copy plasmid DNA purification
Application note available with QIAGEN
Automated use Yes
Brand NucleoSpin
CE certified No, research use only
Elution volume 50 µL
Endotoxin level > 50 EU/µg DNA
Format Mini prep
Gross weight (incl. packaging) 1519.6 g / 3.35 lbs
Handling QIAcube
Hazardous material Yes
Lysate clarification Centrifugation
Packaging dimensions 216 x 216 x 169 mm / 8.50 x 8.50 x 6.65 Inch
Preparation time 20 min/6 preps
Sample amount 1−5 mL (high copy), 6−10 mL (low copy)
Sample material Bacteria, E. coli
Scope of delivery NucleoSpin Plasmid (NoLid) Columns, Collection Tubes, buffers, RNase A
Shelf life (from production) 27 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Silica membrane technology
Theoretical binding capacity 60 µg
Typical downstream application Transfection of sensitive cells and cell injections
Typical purity A260/A280 1.8−1.85
Typical yield 25−45 µg
Vector size < 25 kbp

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